Abstract

Analytical Method Development and Validation for Estimation of Metformin in Bulk and Pharmaceutical Dosage Form by UVSpectrometric Method

Author(s): Mayur Patni1*and Swati Rawat2

A rapid, simple, selective and precise UV-Visible Spectrophotometric method has been developed for the determination of metformin hydrochloride in bulk forms and tablet dosage formulations. The spectrophotome-tric detection was as per carried out at an absorption maximum of 232 nm using phosphate buffer of pH 6.8 as sol-vent. The method was validated for specificity, linearity, accuracy, precision, robustness and ruggedness. The detec-tor response for was linear over the selected concentration range 2 to 12μg/ml with a correlation coefficient of 0.994. The accuracy was carried out as per recovery study and found between 99.1 % to 100.45%. The results demonstrat-ed that the excipients in the tablets did not interfere with the method and can be conveniently employed for routine quality control analysis of metformin in bulk and formulation

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